YinOYang 1.2 Prediction Server The YinOYang WWW server produces neural network predictions for O-ß-GlcNAc attachment sites in eukaryotic protein sequences. This server can also use NetPhos, to mark possible phosphorylated sites and hence identify "Yin-Yang" sites.

Instructions

Output format

Abstract

NetOGlyc

DictyOGlyc

SUBMISSION

Note: Sequences shorter than 21 residues are not recommended. Maximum length: 4000 residues!

Type/paste in your sequence (plaintext, not FASTA) or Swissprot-Acc/ID
OR enter the name of a file with one or more sequences in FASTA format:

File name:

Sequence name: (optional, max 11 chrs.)

Sequence: (plaintext - not FASTA!, only one sequence) Or type in SWISS-PROT ID/AC SignalP is automatically run on all sequences. A warning is displayed if a Signal peptide is detected.

Output format Only positive sites Output for all S/T residues Include Graph "Yin-yang" site predictions (i.e. cross-NetPhos scans) NetPhos threshold

NOTE: All sequences are automatically deleted immediately after prediction

We would appreciate any feedback concerning prediction results

CITATIONS

For publication of results, please cite:

Prediction of glycosylation sites in proteomes: from post-translational modifications to protein function.
R Gupta.
Ph.D. thesis at CBS, 2001.

Prediction of glycosylation across the human proteome and the correlation to protein function.
Gupta, R. and S. Brunak.
Pacific Symposium on Biocomputing, 7:310-322, 2002.

PMID: 11928486         Download the full article in PDF.

ACKNOWLEDGEMENTS

The YinOYang server incorporates results from NetPhos and SignalP.

PORTABLE VERSION

Would you prefer to run YinOYang at your own site? YinOYang 1.2 is available as a stand-alone software package, with the same functionality as the service above. Ready-to-ship packages exist for the most common UNIX platforms. There is a download page for academic users; other users are requested to contact CBS Software Package Manager at software@cbs.dtu.dk.

GETTING HELP